Biochemical Tests for Bacterial Characterization. 1. Hippurate Test
نویسندگان
چکیده
منابع مشابه
Biochemical principle of Limulus test for detecting bacterial endotoxins
A hemocyte lysate from horseshoe crab (Limulus) produced a gel, when exposed to Gram-negative bacterial endotoxins, lipopolysaccharides (LPS). This gelation reaction of the lysate, so-called Limulus test, has been widely employed as a simple and very sensitive assay method for endotoxins. Recent biochemical studies on the principle of Limulus test indicate that the hemocytes contain several ser...
متن کاملRapid biochemical tests for characterization of the Mycoplasmatales.
Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the ...
متن کاملA bacterial chloroform reductive dehalogenase: purification and biochemical characterization
We report herein the purification of a chloroform (CF)-reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23-fold increase in relative purity compared to crude lysate. The membrane fraction obtained by ultracentrifugation was solubilized in Triton X-100 in the presence of glycerol, followed by purifi...
متن کاملMolecular and ultrastructural characterization of porcine hippurate-negative Brachyspira pilosicoli.
Brachyspira pilosicoli, the causative agent of porcine intestinal spirochetosis, usually has hippurate-cleaving capacity. We have regularly isolated hippurate-negative B. pilosicoli from cases of porcine diarrhea. In this study, we show that these biochemically atypical B. pilosicoli isolates can be classified as B. pilosicoli. 16S ribosomal DNA was partially sequenced from eight hippurate-nega...
متن کاملBiochemical Test
This paper explores the potential for the application of neurocomputing technology to the domain of post-operative liver transplant monitoring. The investigation compares a neural network model with two classical statistical techniques using biochemical information obtained from a set of liver transplant patients. Each approach combines the results of a number of liver function tests to predict...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Bulletin of Bacteriological Nomenclature and Taxonomy
سال: 1955
ISSN: 0020-7713,1465-2102
DOI: 10.1099/0096266x-5-3-97